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The active peptide helps you stay away from the tumor

2020-08-14 15:05:26


As of 22 July, the viral epidemic of Novel Coronavirus has affected over 200 countries worldwide and has been confirmed15007683614805People were killed.Vaccines and specific therapies have become important means to control the number of infected people and reduce the death rate, and the development of antiviral drugs is extremely urgent.

Recently, scientists from MIT and other institutions have designed a novel peptide using computer models of protein interactions that bind to coronavirus proteins and break them down through cellular pathways, and this type of peptide could be used as a potential therapy to inhibit SARS-COV-2 virus replication in infected cells.The idea is to use a computer technique to engineer peptides to treat COVID-19, and once they get into cells, they can simply tag and degrade the virus.Now that the researchers have tested the new peptide in human cells, they are planning cell and animal studies to assess its effectiveness.


01


Peptide modeling

Scientists are looking at a number of different strategies to develop novel therapies for COVID-19. One area of interest is developing specific antibodies to bind to inactivated viral proteins such as spikes, which coronavirus USES to enter human cells;One way to do this is to replace antibodies with small fragments of proteins called peptides;MIT researchers are working on engineering of peptide modified to strong combination to the spike protein within the cell, also can use these polypeptides stimulate cell broken viral protein, the researchers of idea is to make the polypeptide recruit called E3 ubiquitin ligase of naturally occurring proteins when the cell no longer needs the protein, it can be marked for destruction.

In order to develop can spike protein peptide, the researchers used protein interactions, a computer model, the model after can optimize the bonding strength between the two kinds of protein, the researchers Chatterjee and others similar computer methods recently used to design for the improvement of gene editing version of the enzymes, the new CRISPR - Cas9 enzymes can be targeted more than 70% of the DNA sequence, and the most common CRISPR - Cas9 can only reach about 10%.In this case, the researchers used the human ACE2 protein, which exists on the surface of specific human cells and binds to coronavirus spike proteins, as a starting point.


Researchers using the developed model will ACE2 protein is broken into many small pieces, then through calculation to predict these fragments may interact with spike protein, they guide the model to optimize the three characteristics, first of all, the engineering modification of peptide has given it to spike protein has the strong affinity, secondly, these peptides can also with other coronavirus spike protein, perhaps can effectively resist the past or the future of other coronavirus strain, third, the researchers said that these peptides are not strong combination with human proteins - to the protein,Under normal circumstances, integrins bind to ACE2 receptors in the human body.This process produces about 25 candidate peptides that can be fused with the E3 ubiquitin ligase and tested in human cells that express spikes (receptor-binding domains, or RBD).


Researchers using the developed model will ACE2 protein is broken into many small pieces, then through calculation to predict these fragments may interact with spike protein, they guide the model to optimize the three characteristics, first of all, the engineering modification of peptide has given it to spike protein has the strong affinity, secondly, these peptides can also with other coronavirus spike protein, perhaps can effectively resist the past or the future of other coronavirus strain, third, the researchers said that these peptides are not strong combination with human proteins - to the protein,Under normal circumstances, integrins bind to ACE2 receptors in the human body.This process produces about 25 candidate peptides that can be fused with the E3 ubiquitin ligase and tested in human cells that express spikes (receptor-binding domains, or RBD).


02


标记被破坏

这种肽类的一个关键优势就是其拥有较小的尺寸,即使与E3泛素连接酶融合,整个链的长度也仅有200个氨基酸左右,研究者认为,编码这种肽类的RNA或DNA可以通过称之为腺相关病毒来进行运输;另一种可能性就是自行运输肽类,让其能与冠状病毒的刺突蛋白结合,并将病毒一同运输到宿主细胞内部,在这种情况下,当病毒一进入细胞就会被标记破坏。

目前研究人员计划在感染SARS-CoV-2病毒的人类细胞中检测这种新型多肽,如果这些测试成功的话,研究人员希望能在动物模型中进行这种多肽的检测,下一步他们还会继续深入研究来改进这种多肽使其能够更好地与刺突蛋白结合并发挥作用。


全球疫情形式依旧紧张,我们仍不能松懈大意,要积极做好疫情防控,戴口罩常通风勤洗手勤消杀万众一心共克时艰!早日打赢这场没有硝烟的战争!

 
 

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